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Catalog Number | orb1294323 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to AKT (pan) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
Dilution range | Western Blot 1:1000-1:2000 Immunofluorescence 1:200-1:500 |
Conjugation | Unconjugated |
Target | Akt1+2+3 / Pan-AKT |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
AKT (pan) antibody at 1/1000 dilution, Lysates at 60 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294323 overnight at 4°C.
AKT (pan) antibody at 1/1000 dilution, Lysates at 60 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294323 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294323 Anti-AKT (pan) antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294323 Anti-AKT (pan) antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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