AKT1 Western Chemiluminescent Blotting Kit Antibody

• Catalog Number: orb345942
• Category: Antibodies
• Description: AKT1 Western Chemiluminescent Blotting Kit
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 14E5.A2.B2.H9
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Isotype: IgG2a
• Dilution range: WB: User Optimized
• Form/Appearance: n/a
• Purity: The kit is designed to detect both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT3 has not been determined. This kit contains sufficient substrate for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.
• Conjugation: Unconjugated
• UniProt ID: P31749
• Storage: See kit insert for complete instructions.
• Buffer/Preservatives: Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
• Alternative names: AKT 1 western blotting, AKT-1, PKB antibody, PKB g
• Note: For research use only
• Application notes: Use Biorbyt’ Anti-AKT1 Chemiluminescent Kit for Western Blotting for detection of human AKT1 native and recombinant proteins by western blot. Expect a band approximately 56 kDa in size corresponding to AKT1 protein. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.
• Expiration Date: Please enquire.

Western blot analysis of His-Tagged Recombinant using AKT1 Western Chemiluminescent Blotting Kit