AKT1 (Phospho-T450) antibody

• Catalog Number: orb393200
• Category: Antibodies
• Description: Rabbit polyclonal antibody to AKT1 (Phospho-T450)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat, Sheep
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T450 of human AKT protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: AKT1
• Entrez: 207, 11651, 24185
• UniProt ID: P47196, P31750, P31749
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: PKB; RAC; RAC-alpha serine/threonine-protein kinas
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AKT (Phospho-T450) expression in HEK293T-starve treated (A), A549-EGF treated (B), A549-PMA treated (C), mouse liver (D), mouse lung (E), rat liver (F), rat lung (G) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 60 kD)

Immunohistochemical analysis of AKT (Phospho-T450) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.