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Catalog Number | orb2296231 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a full length recombinant AKT1. |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | IgG2a Lambda |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Immunogen | AKT1 (AAH00479.1, 1 a.a. ~ 480 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Protein Sequence | MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTLHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA |
Tested applications | ELISA, IF, WB |
Clone Number | 6F11 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
NCBI | AAH00479.1 |
AKT1 monoclonal antibody (M09), clone 6F11 Western Blot analysis of AKT1 expression in Jurkat.
AKT1 monoclonal antibody (M09), clone 6F11. Western Blot analysis of AKT1 expression in NIH/3T3.
AKT1 monoclonal antibody (M09), clone 6F11. Western Blot analysis of AKT1 expression in PC-12.
AKT1 monoclonal antibody (M09), clone 6F11. Western Blot analysis of AKT1 expression in Raw 264.7.
Detection limit for recombinant GST tagged AKT1 is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged AKT1 is approximately 10 ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (78.54 KDa).