AKT1 Antibody (C-term T450)

• Catalog Number: orb1937820
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB29255
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 55686 Da
• Target: This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-457 amino acids from the C-terminal region of human AKT1.
• UniProt ID: P31749
• NCBI: NP_005154.2, NP_001014432.1, NP_001014431.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: RAC-alpha serine/threonine-protein kinase, Protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

AKT1 Antibody (C-term T450) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

AKT1 Antibody (C-term T450) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of AKT1 Antibody (C-term T450) for immunohistochemistry. Clinical relevance has not been evaluated.

AKT1 Antibody (pT450) western blot analysis in MCF-7 cell line lysates (35 ug/lane). This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).

AKT1 Antibody (pT450) western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).

Confocal immunofluorescent analysis of AKT1 Antibody (C-term T450) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).