You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb764338 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Akt (phospho-Thr308) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Akt (phospho Thr308) |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 55 |
Storage | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-AKT1 antibody, anti-PKB antibody, anti-RAC an Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of lysates from A431 and Hela cells treated with UV or insulin 0.01U/ml, using AKT p-308 Antibody. Primary Antibody was diluted at 1:1000 4°C over night, secondary antibody was diluted at 1:10000, 37° 1hour.
Immunofluorescence analysis of human-lung tissue. 1, Akt (phospho Thr308) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunofluorescence analysis of human-lung tissue. 1, Akt (phospho Thr308) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunofluorescence analysis of human-stomach tissue. 1, Akt (phospho Thr308) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
FC, ICC, IHC-P, WB | |
Bovine, Canine, Gallus, Porcine, Rabbit, Sheep |
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Canine, Gallus, Porcine, Rabbit, Sheep |
Filter by Rating