Akt (phospho-pT308) antibody

• Catalog Number: orb344405
• Category: Antibodies
• Description: Akt (phospho-pT308) antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 18F3.H11
• Tested applications: ELISA, FC, IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: Anti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000, FC: User Optimized, IHC: 20 µg/ml, IF: 1:25, IP: User Optimized, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-AKT pT308 antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur.
• Conjugation: Unconjugated
• UniProt ID: P31749
• NCBI: 62241011
• Storage: Store Anti-AKT pT308 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-AKT pT308 Antibody, RAC-PK-alpha, Prote
• Note: For research use only
• Application notes: Anti-AKT pT308 antibody is tested for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pT308 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human brain cerebellum tissue using Akt (phospho-pT308) antibody

Western blot analysis of non-phosphorylated AKT in untreated cells (Lane1), phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates (Lane2) using Akt (phospho-pT308) antibody