AKT ISOFORM antibody

• Catalog Number: orb345936
• Category: Assays and Kits
• Description: AKT ISOFORM antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: All Anti-AKT isoform specific Antibodies were produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1, AKT2 or AKT3 protein.
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:2,000 - 1:10,000, FC: User Optimized, IHC: 20 µg/mL, WB: 1:1000
• Form/Appearance: Liquid: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Purity: This primary antibody kit has been carefully chosen to recognize all 3 isoforms of AKT. Anti-AKT1 antibody is directed against human AKT1. Anti-AKT2 antibody is directed against human AKT2. Anti-AKT3 antibody is directed against human AKT3. The antibodies detect both unphosphorylated and phosphorylated forms of the protein. The antibodies were purified from ascites by Protein A chromatography.
• Conjugation: Unconjugated
• UniProt ID: P31749
• Storage: Each vial contains a relatively low volume of reagent (25 µL). Store vial at -20° C or below prior to opening. To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: AKT isoform antibody kit, AKT-1, AKT-2, AKT-3, Pro
• Note: For research use only
• Application notes: Anti-AKT Antibodies are suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT proteins by western blotting in the appropriate cell lysate or extract. All 3 primary monoclonal antibodies reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required. This sampler kit consists of sample sizes of anti-AKT1, anti-AKT2 and anti-AKT3 mouse monoclonal antibody and HRP conjugated anti-Mouse IgG secondary antibody (p/n 200-301-I51S; 200-301-BR8S; 200-301-J36S and 610-403-C46S, respectively).
• Expiration Date: Please enquire.

Western blot analysis of His-Tagged Recombinant using AKT ISOFORM antibody

Western blot analysis of using AKT ISOFORM antibody

Western blot analysis of His-Tagged Recombinant using AKT ISOFORM antibody