AKT1 Antibody

SKU: orb344458

Description

AKT antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000, IHC: 20 ug/ml, WB: 1:500 - 1:3,000
• Reactivity: Mouse
• Application Notes:
  Anti-AKT monoclonal antibody has been tested by ELISA and western blotting. This antibody is suitable in immunohistochemistry and immunoprecipitation. Expect a band approximately 56 kDa in size corresponding to AKT protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human and mouse AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 14E5.16C8.25F6
• Immunogen: Anti-Akt monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human AKT1 protein.
• Target: AKT1
• Purity: This antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein. A BLAST analysis was used to suggest cross-reactivity with AKT from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store Akt Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-AKT Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates (p/n orb348714). Lane 2: PDGF stimulated NIH/3T3 cell lysates (p/n orb348723). Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n orb347385) was used at a 1:40000 dilution for 1 h at 4°C with FemtoMax™ enhanced chemiluminescent reagent. Block: 5% BLOTTO (p/n orb348624) in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.

Quick Database Links

Gene Symbol

AKT1

UniProt

P31749

RefSeq:62241011