AKR1C4 monoclonal antibody (M01), clone 2C11

• Catalog Number: orb2294462
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant AKR1C4.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2C11
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: AKR1C4 (AAH20744, 1 a.a. ~ 323 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MDPKYQRVELNDGHFMPVLGFGTYAPPEVPRNRAVEVTKLAIEAGFRHIDSAYLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCTFFQPQMVQPALESSLKKLQLDYVDLYLLHFPMALKPGETPLPKDENGKVIFDTVDLSATWEVMEKCKDAGLAKSIGVSNFNYRQLEMILNKPGLKYKPVCNQVECHPYLNQSKLLDFCKSKDIVLVAHSALGTQRHKLWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIRENIQVFEFQLTSEDMKVLDGLNRNYRYVVMDFLMDHPDYPFSDEY
• NCBI: AAH20744
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

AKR1C4 monoclonal antibody (M01), clone 2C11 Western Blot analysis of AKR1C4 expression in MCF-7.

Detection limit for recombinant GST tagged AKR1C4 is 3 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to AKR1C4 on HepG2 cell. [antibody concentration 15 ug/ml].

Immunoperoxidase of monoclonal antibody to AKR1C4 on formalin-fixed paraffin-embedded human liver tissue. [antibody concentration 3 ug/ml].

Western Blot analysis of AKR1C4 expression in transfected 293T cell line by AKR1C4 monoclonal antibody (M01), clone 2C11. Lane 1: AKR1C4 transfected lysate(37.2 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (61.27 KDa).