AK2 Antibody

• Catalog Number: orb381036
• Category: Antibodies
• Description: AK2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human AK2 recombinant protein (Position: E161-I239). Human AK2 shares 93.7% and 93% amino acid (aa) sequence identity with mouse and rat AK2, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 26478 MW
• UniProt ID: P54819
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Adenylate kinase 2, mitochondrial ;AK 2 ;2.7.4.3 ;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-AK2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of AK2 using anti-AK2 antibody.Lane 1:human HeLa cell; 2:human HL-60 cell; 3:human 293T cell; 4:human HepG2 cell; 5:rat heart tissue; 6:rat liver tissue; 7:mouse heart tissue; 8:mouse liver tissue.

IF analysis of AK2 using anti-AK2 antibody. AK2 was detected in immunocytochemical section of U20S cells.

IHC analysis of AK2 using anti-AK2 antibody. AK2 was detected in a paraffin-embedded section of mouse kidney tissue.

IHC analysis of AK2 using anti-AK2 antibody. AK2 was detected in a paraffin-embedded section of rat liver tissue.

IHC analysis of AK2 using anti-AK2 antibody. AK2 was detected in a paraffin-embedded section of human lung cancer tissue.