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Catalog Number | orb19562 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to AIP |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:64000, WB: 0.02-0.06 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 37.7 |
Target | AIP / ARA9 |
Entrez | 9049 |
Protein Sequence | EARIRQKDEEDKAR |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti AIP antibody, anti ARA9 antibody, anti aryl h Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 37kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 37.7kDa according to Human NP_003968.1 and 37.6kDa according to mouse NP_057875.1). In transfected HEK293 transiently expressing ARA9 a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.02-0.06 μg/ml. |
Expiration Date | 12 months from date of receipt. |
HEK293 overexpressing ARA9 (RC211157) and probed with orb19562 (mock transfection in first lane), tested by Origene.
Western blot analysis of NIH/3T3 cell lysate using AIP antibody
EB07776 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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