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AHA1 Antibody: PerCP

Catalog Number: orb147674

DispatchUsually dispatched within 2-3 weeks
$ 610.00
Catalog Numberorb147674
CategoryAntibodies
DescriptionRat monoclonal to Aha1 (PerCP). Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding. It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors..
TargetAHA1
ClonalityMonoclonal
Species/HostRat
IsotypeIgG2a Kappa
ConjugationPerCP
ReactivityHuman, Mouse, Rat
Concentration1.2 mg/ml
Buffer/Preservatives95.64mM Phosphate, 2.48mM MES and 2mM EDTA
ImmunogenRecombinant Full Length Mouse Aha1 Protein
UniProt IDQ8BK64
MW38kDa
Tested applicationsELISA, ICC, IF, IHC, WB
Dilution rangeWB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000)
Application notes1 µg/ml of SMC-172 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Clone Number25F2.D9
StorageConjugated antibodies should be stored according to the product label
Alternative namesAha 1 antibody, Ahsa1 antibody, p38 antibody, HSPC
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NoteFor research use only
Entrez217737
NCBINP_666148.1
AHA1 Antibody: PerCP

Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.

AHA1 Antibody: PerCP

Immunoprecipitation analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000.

AHA1 Antibody: PerCP

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: Cervical cancer cell line (HeLa). Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).

AHA1 Antibody: PerCP

Western Blot analysis of Human Cell lysates showing detection of Aha1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

  • AHA1 Antibody: PerCP [orb147691]

    ELISA,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rat

    Monoclonal

    PerCP

    100 μg
  • AHA1 Antibody: PerCP [orb152019]

    ELISA,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    PerCP

    100 μl