AGPS antibody

• Catalog Number: orb179854
• Category: Antibodies
• Description: Mouse monoclonal antibody to AGPS.
• Clonality: Monoclonal
• Clone Number: AGPS-03
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: recombinant human AGPS (amino acids 158-384)
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: AGPS
• Entrez: 8540
• UniProt ID: O00116
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-ADAS antibody, anti-ADPS antibody, anti-RCDP3
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of K562 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with K562 cells stained using anti-AGPS (AGPS-03) purified antibody (concentration in sample 5 µg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with K562 cells stained using anti-AGPS (AGPS-03) purified antibody (concentration in sample 5 µg/ml, GAM APC).

Western blotting analysis of human AGPS using mouse monoclonal antibody AGPS-03 on lysates of HEP-G2 and Molt-4 cells, and MCF-7 cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-AGPS monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. AGPS was detected at approximately 70 kDa.