AGK antibody

• Catalog Number: orb19591
• Category: Antibodies
• Description: Goat polyclonal antibody to AGK
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 47.1
• Target: Acylglycerol kinase
• Entrez: 55750
• Protein Sequence: CDPRKREQMLTSP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti acylglycerol kinase antibody, anti FLJ10842 a
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 50kDa band observed in Human Brain (Substantia nigra, Hippocampus) lysates (calculated MW of 47.1kDa according to NP_060708.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19591 (1ug/ml) staining of Human Brain (Substantia nigra) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.