AGAP3 antibody

• Catalog Number: orb18777
• Category: Antibodies
• Description: Goat polyclonal antibody to AGAP3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 1ug/ml, IHC-P: 5-10µg/ml
• Conjugation: Unconjugated
• MW: 98
• Target: CENTG3 / MRIP1
• Entrez: 116988
• Protein Sequence: NPSAELHRSPSLL
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti AGAP3 antibody, anti CENTG3 antibody, anti MR
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows strong staining of brush borders in Proximal Convoluted tubules. Recommended concentration, 5-10ug/ml.WB: Preliminary experiments gave an approx 45kDa band in Human Brain and Human Liver lysates at 1 μg/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 101kDda according to NP_114152. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb18777 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.