AF6 Antibody

• Catalog Number: orb1527033
• Category: Antibodies
• Description: AF6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1475 and 1525
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: AF6
• UniProt ID: P55196
• NCBI: BAA32484.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: l-afadin; protein AF-6; myeloid/lymphoid or mixed-
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse AF6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AF6 antibody orb1527033 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human AF6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AF6 antibody orb1527033 used for IP at 6 µg per reaction.