AF-10 antibody

• Catalog Number: orb764484
• Category: Antibodies
• Description: Rabbit polyclonal antibody to AF-10
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human AF10. AA range:211-260
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 109
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-MLLT10 antibody, anti-AF10 antibody, anti-Pro
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Hela cell. 1, AF-10 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). COX IV Monoclonal Antibody (6C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of various cells using AF-10 Polyclonal Antibody diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of lysates from COLO205 and Jurkat cells, using AF10 Antibody. The lane on the right is blocked with the synthesized peptide.