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Catalog Number | orb1257122 |
---|---|
Category | Antibodies |
Description | ADSL Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human ADSL (NP_000017.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:20 - 1:100IP: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 55kDa |
Target | ADSL |
UniProt ID | P30566 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | AMPS, ASASE, ASL, adenylosuccinate lyase, adenylos Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:20 - 1:100IP: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using ADSL antibody (orb1257122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using ADSL antibody (orb1257122). Blue: DAPI for nuclear staining.
IHC-P, WB | |
Bovine, Canine, Equine, Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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