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ADRB2 Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb1499374

DispatchUsually dispatched within 5-10 working days
$ 270.00
Catalog Numberorb1499374
CategoryAntibodies
DescriptionADRB2 Recombinant Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityRecombinant
Clone Number7C1
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityMouse, Rat, Zebrafish
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human ADRB2
Antibody TypeRecombinant Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
Form/AppearanceLiquid
ConjugationUnconjugated
MW60 kDa
TargetADRB2
UniProt IDP07550
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesbeta 2-adrenergic receptor; beta 2 Adrenergic Rece
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NoteFor research use only
Expiration Date12 months from date of receipt.
ADRB2 Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Mouse kidney, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 46 kDa, Observed MW: 46 kDa.

ADRB2 Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: A431, 2: MCF-7, 3: MDA-MB-231, 4: Rat kidney, 5: Mouse kidney, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 46 kDa, Observed MW: 46 kDa.

ADRB2 Recombinant Rabbit Monoclonal Antibody

Cell line: A431, Fixation: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1499374.

ADRB2 Recombinant Rabbit Monoclonal Antibody

Cell line: MCF7, Fixation: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1499374.

ADRB2 Recombinant Rabbit Monoclonal Antibody

Cell line: SH-SY5Y, Fixation: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4°C, overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue).

ADRB2 Recombinant Rabbit Monoclonal Antibody

Cell line: SH-SY5Y, Fixation: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary Ab Dilution: 1:100, Secondary Ab: Goat Anti-Rabbit IgG, Unlabelled control: The cell without incubation, with primary antibody and secondary antibody, (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1499374.

ADRB2 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-beta 2 Adrenergic Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1499374, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ADRB2 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-beta 2 Adrenergic Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1499374, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ADRB2 Recombinant Rabbit Monoclonal Antibody

Western blot analysis of beta 2 Adrenergic Receptor on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (orb1499374, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.

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