ADO Antibody

• Catalog Number: orb381034
• Category: Antibodies
• Description: ADO Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 29751 MW
• UniProt ID: Q96SZ5
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: 2-aminoethanethiol dioxygenase;1.13.11.19;Cysteami
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-ADO antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U251 cells using anti-ADO antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of HeLa cells using anti-ADO antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of PC-3 cells using anti-ADO antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ADO using anti-ADO antibody.Lane 1:rat brain tissue;2:rat testicular tissue;3:rat spleen tissue;4:human MCF-7 cell;5:human SW620 cell;6:mouse spleen tissue;7:mouse heart tissue;8:mouse HEPA1-6 cell;9:mouse SP2/0 cell.

IF analysis of ADO using anti-ADO antibody. ADO was detected in immunocytochemical section of A549 cells.

IHC analysis of ADO using anti-ADO antibody. ADO was detected in paraffin-embedded section of rat brain tissue.

IHC analysis of ADO using anti-ADO antibody. ADO was detected in paraffin-embedded section of mouse brain tissue.