ADM Antibody

• Catalog Number: orb1264358
• Category: Antibodies
• Description: ADM Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ADM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-96 amino acids from the Central region of human ADM.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:2000For FACS starting dilution is: 1:25
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 20 kDa
• Target: ADM
• UniProt ID: P35318
• NCBI: P35318
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: ADM, Adrenomedullin, AM, Proadrenomedullin N-20 te
• Note: For research use only
• Application notes: For WB starting dilution is: 1:2000For FACS starting dilution is: 1:25
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western Blot at 1:1000-1:2000 dilution Lane 1: human breast lysate Lane 2: human kidney lysate Lane 3: human lung lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of ADM Antibody in mouse lung tissue lysates (35 ug/lane)

Flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.