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Catalog Number | orb381033 |
---|---|
Category | Antibodies |
Description | ADK Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human ADK recombinant protein (Position: K165-T351). Human ADK shares 88.8% and 88.2% amino acid (aa) sequence identity with mouse and rat ADK, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 40545 MW |
UniProt ID | P55263 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Adenosine kinase;AK;2.7.1.20;Adenosine 5'-phosphot Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of ADK using anti-ADK antibody.Lane 1:human placenta tissue; 2:human PC-3 cell; 3:human T47D cell; 4:human U937 cell; 5:human U251 cell; 6:human HepG2 cell.
WB analysis of ADK using anti-ADK antibody.Lane 1:rat liver tissue; 2:rat kidney tissue; 3:rat stomach tissue; 4:rat PC-12 cell; 5:mouse kidney tissue; 6:mouse stomach tissue; 7:mouse NIH/3T3 cell.
IF analysis of ADK using anti-ADK antibody. ADK was detected in an immunocytochemical section of PC-3 cells.
IHC analysis of ADK using anti-ADK antibody. ADK was detected in a paraffin-embedded section of human placenta tissue.
IHC analysis of ADK using anti-ADK antibody. ADK was detected in a paraffin-embedded section of mouse liver tissue.
IHC analysis of ADK using anti-ADK antibody. ADK was detected in a paraffin-embedded section of rat liver tissue.
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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