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Item 1 of 4
Item 1 of 4
Adenosine A2 beta Receptor Antibody
Catalog Number: orb1247841
| Catalog Number | orb1247841 |
|---|---|
| Category | Antibodies |
| Description | Adenosine A2 beta Receptor Antibody |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, FC, IF, IHC |
| Reactivity | Human |
| Immunogen | The immunogen for this antibody is: CQADVKSGNGQ |
| Antibody Type | Primary Antibody |
| Concentration | 500 ug/mL |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| Target | ADORA2B |
| UniProt ID | P29275 |
| NCBI | NP_000667.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | ADORA2B, adenosine A2b receptor, HGNC:264, ADORA2, Read more... |
| Note | For research use only |
| Application notes | Peptide ELISA: antibody detection limit dilution 1:64000.Immunohistochemistry:In paraffin embedded Human Kidney shows cytoplasm staining in cells of renal tubules. Recommended concentration: 2-4ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml. |
| Expiration Date | 12 months from date of receipt. |
orb1247841 (2 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
orb1247841 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.
orb1247841 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.
orb1247841 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line).
