Actin gamma-cytoplasmic antibody

• Catalog Number: orb98202
• Category: Antibodies
• Description: Mouse monoclonal to Actin gamma-cytoplasmic
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2A3
• Tested applications: ELISA, FC, IHC-Fr, IHC-P, WB
• Reactivity: Gallus, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
• Isotype: IgG2b
• Form/Appearance: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• UniProt ID: P63261
• Source: 2A3 is a Mouse monoclonal IgG2b antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of γ-cytoplasmic actin with an acetylated N-terminus (Ac-EEEIAALVI-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue. The antibody does not cross react with other actin isoforms.
• Storage: Storage: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product
• Buffer/Preservatives: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Methanol fixed human dermal fibroblasts immunostained with orb98202 (2A3; 1:500)

Reactivity of orb98202 (up to a 1:1000 dilution) in the epithelial villi and smooth muscle cells in swine colon.