Actin antibody

• Catalog Number: orb44724
• Category: Antibodies
• Description: Mouse Monoclonal to Actin.
• Clonality: Monoclonal
• Clone Number: HHF35
• Tested applications: ICC, IHC-Fr, IHC-P, WB
• Reactivity: Canine, Feline, Gallus, Human, Mouse, Primate, Rabbit, Rat
• Isotype: Mouse IgG1
• Immunogen: SDS extracted protein fraction of human myocardium
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 1 μg/ml; positive control: murine femoral muscle, murine heart, negative control: HUVEC line; reducing conditions. Immunohistochemistry (paraffin sections): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Actin (muscle)
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Actin antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1 μg/ml; positive control: murine femoral muscle, murine heart, negative control: HUVEC line; reducing conditions. Immunohistochemistry (paraffin sections): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of muscle-specific actin using the monoclonal antibody HHF35. Lane 1: murine femoral muscle; Lane 2: murine heart; Lane 3: HeLa; Lane 4: HUVEC.

Immunohistochemistry staining of human muscle (paraffin-embedded sections) with anti-muscle-specific actin (HHF35).