Actin, alpha skeletal muscle Mouse Monoclonal Antibody

SKU: orb500988

Description

Actin, alpha skeletal muscle Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:2000-5000, IHC-P=1:200-800, IHC-F=1:200-800, IF=1:200-800
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Duck

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 5F11
• Immunogen: KLH conjugated synthetic peptide derived from human ACTA1
• Target: ACTA1
• Molecular Weight: 42 kDa
• Purification: Affinity purified by Protein G

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ACTS_HUMAN; Actin, alpha skeletal muscle; alpha-actin-1; ACTA1; ACTA; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; Actin alpha skeletal muscle; actin, alpha 1, skeletal muscle 1; actin, alpha 1, skeletal muscle; actina; actine; aktin; alpha Actin 1; alpha skeletal muscle Actin; alpha skeletal muscle; alpha-actin; beta cytoskeletal actin; nemaline myopathy type 3.

Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat uterine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: LOVO Cell (Human) Lysate at 40 ug, Primary: Anti-Actin, alpha skeletal muscle (orb500988) at 1/2 000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 47 kD.

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Actin, alpha skeletal muscle (orb500988) at 1/2 000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 47 kD.

Quick Database Links

Gene Symbol

ACTA1

UniProt

P68133