Actin, alpha skeletal muscle Mouse Monoclonal Antibody

SKU: orb1227215

Description

Actin, alpha skeletal muscle Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:200-800, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Mouse, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 3E9
• Immunogen: KLH conjugated synthetic peptide derived from human ACTA1
• Target: ACTA1
• Molecular Weight: 42 kDa
• Purification: Affinity purified by Protein G

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ACTS_HUMAN; Actin, alpha skeletal muscle; alpha-actin-1; ACTA1; ACTA; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; Actin alpha skeletal muscle; actin, alpha 1, skeletal muscle 1; actin, alpha 1, skeletal muscle; actina; actine; aktin; alpha Actin 1; alpha skeletal muscle Actin; alpha skeletal muscle; alpha-actin; beta cytoskeletal actin; nemaline myopathy type 3.

25 ug total protein per lane of various lysates probed with Actin, alpha skeletal muscle monoclonal antibody, unconjugated (orb1227215) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated (orb1227215) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated (orb1227215) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Quick Database Links

Gene Symbol

ACTA1

UniProt

P68133