Actin alpha 1 Antibody

• Catalog Number: orb1244248
• Category: Antibodies
• Description: Actin alpha 1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human Actin alpha 1.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 103 kDa
• Target: ACTN1
• UniProt ID: P12814
• NCBI: NP_001093
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: ACTN1, FLJ40884, BDPLT15
• Note: For research use only
• Application notes: Actin alpha 1 antibody can be used for detection of Actin alpha 1 by ELISA at 1:312500. Actin alpha 1 antibody can be used for detection of Actin alpha 1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Expiration Date: 12 months from date of receipt.

Antibody used in WB on Rat Cortical Neurons at 1:1000.

Antibody used in WB on Human 293T at 0.2-1 ug/ml.

Antibody used in WB on Human HEK293 at 1:1000.

Antibody used in WB on rACTNX-GFP at 1:1000 (Lane 1: 10 ug ACTN1-GFP transfected COS-7 lysate, Lane 2: 10 ug ACTN2-GFP transfected COS-7 lysate, Lane 3: 10 ug ACTN3-GFP transfected COS-7 lysate, Lane 4: 10 ug ACTN4-GFP transfected COS-7 lysate).

Antibody used in IHC on rACTNX-GFP at 1:1000.