ACTH (Adrenocorticotrophic Hormone) (Adrenal Marker) Antibody

• Catalog Number: orb2318532
• Category: Antibodies
• Description: ACTH (Adrenocorticotrophic Hormone) (Adrenal Marker) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM333
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Synthetic peptide corresponding to aa1-24 of human ACTH
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: ELISA (For coating, order Ab without BSA); Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No special pretreatment is required for staining of formalin-fixed tissues)Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: ACTH is ~5kDa|and the POMC precursor is ~30kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.
• Target: POMC
• Entrez: 5443
• UniProt ID: P01189
• Expression System: CHO cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Adrenocorticotropin; alpha or beta or gamma Melano
• Note: For research use only
• Application notes: Positive Control: Normal pituitary gland or pituitary tumor
• Expiration Date: 12 months from date of receipt.