ACTB Antibody

• Catalog Number: orb1240312
• Category: Antibodies
• Description: ACTB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: b-actin antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human b-actin.
• Concentration: 1 mg/mL
• Dilution range: b-actin antibody can be used for the detection of b-actin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ACTB
• UniProt ID: P60709
• NCBI: AAH02409
• Storage: beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: beta-Actin Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: beta-Actin Antibody: Beta actin, b actin
• Note: For research use only
• Application notes: b-actin antibody can be used for the detection of b-actin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of beta-Actin in A431, Daudi, HepG2, HL60, Jurkat, Human brain, Mouse lung, and Chicken liver lysate with beta-Actin antibody at 1 µg/mL.

Immunocytochemistry of Actin in HeLa cells with Actin antibody at 1 µg/mL.

Immunofluorescence of beta-Actin in Hela cells with beta-Actin antibody at 2 µg/mL.