ACTB Antibody

• Catalog Number: orb1240116
• Category: Antibodies
• Description: ACTB Antibody
• Species/Host: Gallus
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, WB
• Predicted Reactivity: Bovine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgY
• Immunogen: Beta-actin antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human beta-actin.The immunogen is located within amino acids 90 - 140 of Beta-actin.
• Concentration: 1 mg/mL
• Dilution range: beta-actin antibody can be used for the detection of beta-actin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ACTB
• UniProt ID: P60709
• NCBI: AAH02409
• Storage: Beta-actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Beta-actin Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Beta-actin Antibody: BRWS1, PS1TP5BP1, Actin, cyto
• Note: For research use only
• Application notes: beta-actin antibody can be used for the detection of beta-actin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of actin in HeLa cell lysate with b-actin antibody at (A) 1 and (B) 2 µg/mL.

Immunocytochemistry of Actin in HeLa cells with Actin antibody at 2 µg/mL.