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Catalog Number | orb381027 |
---|---|
Category | Antibodies |
Description | ACSL1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human ACSL1 recombinant protein (Position: D604-V698). Human ACSL1 shares 81.1% and 86.3% amino acid (aa) sequence identity with mouse and rat ACSL1, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 77943 MW |
UniProt ID | P33121 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Long-chain-fatty-acid--CoA ligase 1;6.2.1.3;Acyl-C Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A431 cells using anti-ACSL1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of ACSL1 using anti-ACSL1 antibody.Lane 1:human HepG2 cell; 2:human U87 cell; 3:rat liver tissue; 4:rat heart tissue; 5:mouse liver tissue.
IF analysis of ACSL1 using anti-ACSL1antibody. ACSL1 was detected in immunocytochemical section of U20S cells.
IHC analysis of ACSL1 using anti-ACSL1 antibody. ACSL1 was detected in a paraffin-embedded section of mouse kidney tissue.
IHC analysis of ACSL1 using anti-ACSL1 antibody. ACSL1 was detected in a paraffin-embedded section of rat kidney tissue.
IHC analysis of ACSL1 using anti-ACSL1 antibody. ACSL1 was detected in a paraffin-embedded section of human liver cancer tissue.
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, WB | |
Guinea pig | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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