ACK (phospho-Tyr284) antibody

• Catalog Number: orb767309
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ACK (phospho-Tyr284)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human ACK1 around the phosphorylation site of Tyr284. AA range:250-299
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 120
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-TNK2 antibody, anti-ACK1 antibody, anti-Activ
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from HepG2 cells, treated with EGF 200ng/ml 30', (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ACK1 (Phospho-Tyr284) Antibody.

Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is blocked with the phospho peptide.