Acidic Cytokeratin Antibody [AE1]

• Catalog Number: orb1252668
• Category: Antibodies
• Description: Acidic Cytokeratin Antibody [AE1]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: AE1
• Tested applications: FC, IF, IHC, WB
• Reactivity: Bovine, Canine, Gallus, Human, Monkey, Mouse, Rabbit, Rat, Reptile
• Isotype: IgG1
• Immunogen: Solubilized keratin extract from human stratum corneum
• Antibody Type: Primary Antibody
• Concentration: 0.2 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: KRT10
• UniProt ID: P13645
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Keratin, type I cytoskeletal 10, Cytokeratin-10, C
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Acidic Cytokeratin antibody AE1 to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of human skin with Acidic Cytokeratin antibody AE1.

Western blot testing using Acidic Cytokeratin antibody AE1.

SDS-PAGE Analysis of Purified, BSA-Free Acidic Cytokeratin Antibody (clone AE1). Confirmation of Integrity and Purity of the Antibody.