KRT10 Antibody

SKU: orb1252668

Description

KRT10 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Bovine, Canine, Gallus, Human, Monkey, Mouse, Rabbit, Rat, Reptile
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 µg/mLWB: 0.5-1 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Acidic Cytokeratin antibody AE1 to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: AE1
• Immunogen: Solubilized keratin extract from human stratum corneum
• Target: KRT10
• Purification: Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KRT10, KPP

IHC staining of human skin with Acidic Cytokeratin antibody AE1.

Western blot testing using Acidic Cytokeratin antibody AE1.

SDS-PAGE Analysis of Purified, BSA-Free Acidic Cytokeratin Antibody (clone AE1). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

KRT10

UniProt

P13645