Acetylcholinesterase antibody (Peroxidase)

• Catalog Number: orb344265
• Category: Antibodies
• Description: Acetylcholinesterase antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Bovine
• Isotype: IgG
• Immunogen: Acetylcholinesterase [Bovine Erythrocytes]
• Concentration: 1 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:2,500
• Form/Appearance: Lyophilized
• Purity: Anti-Acetylcholinesterase (Bovine Erythrocytes) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Acetylcholinesterase [Bovine Erythrocytes]. Cross reactivity against Acetylcholinesterase from other sources is unknown.
• Conjugation: HRP
• UniProt ID: P23795
• NCBI: NP_001069688.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: goat anti-Acetyl Cholinesterase Antibody, HRP conj
• Note: For research use only
• Application notes: Anti-Acetylcholinesterase (Bovine Erythrocytes) antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Acetylcholinesterase [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.