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Catalog Number | orb1927687 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB24908 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 74354 Da |
Target | This ACBG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-265 amino acids from the Central region of human ACBG2. |
UniProt ID | Q5FVE4 |
NCBI | NP_112186.3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Long-chain-fatty-acid--CoA ligase ACSBG2, Acyl-CoA Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of ACBG2 Antibody (Center) in K562 cell line lysates (35 ug/lane). ACBG2 (arrow) was detected using the purified Pab.
ACBG2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-ACBG2 Antibody (Center) at 1:1000 dilution + human testis lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 74 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
ACBG2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACBG2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.