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Catalog Number | orb1254947 |
---|---|
Category | Antibodies |
Description | ACAN Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 350-450 of human ACAN (NP_037359.3). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:1000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 230kDa |
Target | ACAN |
UniProt ID | P16112 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | ACAN, AGCAN, Aggrecan core protein, Acg1, Aggrecan Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:1000 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of mouse brain, using ACAN antibody (orb1254947) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
IF, IH, WB | |
Human, Mouse, Porcine, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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