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Catalog Number | orb532361 |
---|---|
Category | Tools |
Description | The tetraacetylated N-Azidoacetyl-mannosamine (Ac 4ManNAz) provides a non-radioactive alternative for glycoconjugate vi-sualization. It is cell-permeable, intracellularly processed and incorporated instead of its natural monosaccharide counterpart N-Acetylmannosamine(ManNAc). The resulting Azide-functionalized glycoconjugates can subse-quently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Azides of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging. Recommended concentration for metabolic labeling: 25-75 µM. This concentration range may serve as a starting point for an individual assay set-up. |
CAS Number | 361154-30-5 |
Form/Appearance | oil; Color: off-white to grey |
Purity | mass identification (ESI-MS) |
MW | Theoretical MW: 430.37 g/mol; Detected MW: 430.13 g/mol |
SMILES | [C@H]1([C@@H](C(O[C@@H]([C@H]1OC(=O)C)COC(=O)C)OC(=O)C)NC(=O)CN=[N+]=[N-])OC(=O)C |
Formula | C16H22N4O10 |
Solubility (25°C) | DMSO, DMF, MeOH |
Storage | store at -20 °C |
Note | For research use only |
Application notes | Glycoconjugate synthesis monitoring by metabolic labeling. |
Expiration Date | 12 months from date of receipt. |
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