ABCG8 Antibody

• Catalog Number: orb614092
• Category: Antibodies
• Description: ABCG8 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ABCG8 recombinant protein (Position: R50-D672).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human, Rat Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 76 kDa
• UniProt ID: Q9H221
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: ATP-binding cassette sub-family G member 8; Sterol
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IF analysis of ABCG8 using anti-ABCG8 antibody.

Flow Cytometry analysis of RH35 cells using anti-ABCG8 antibody(Blue line).The cells were blocked with 10% normal goat serum.And then incubated with rabbit anti-ABCG8.

Flow Cytometry analysis of HepG2 cells using anti-ABCG8 antibody(Blue line).The cells were blocked with 10% normal goat serum.And then incubated with rabbit anti-ABCG8

WB analysis using anti-ABCG8 antibody.Lane 1:A431 cell, Lane 2:rat small intestine tissue, Lane 3:mouse liver tissue, Lane 4:rat RH35 cell