ABCG5 Antibody

• Catalog Number: orb96998
• Category: Antibodies
• Description: ABCG5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG5 (116-131aa NGRALRREQFQDCFSY).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, HumanImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 72504 MW
• UniProt ID: Q9H222
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: ATP-binding cassette sub-family G member 5;Steroli
• Note: For research use only
• Application notes: WB: The detection limit for ABCG5 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: MCF-7 Cell Lysate, Lane 2: A549 Cell Lysate, Lane 3: HT1080 Cell Lysate, Lane 4: U87 Cell Lysate, Lane 5: SKOV Cell Lysate using ABCG5 antibody