ABCB11 Antibody

• Catalog Number: orb259591
• Category: Antibodies
• Description: ABCB11 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ABCB11 (1175-1199aa KYGDNTKEIPMERVIAAAKQAQLHD), different from the related mouse and rat sequences by three amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunofluorescence, 2μg/ml, Mouse Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 146407 MW
• UniProt ID: O95342
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Bile salt export pump;ATP-binding cassette sub-fam
• Note: For research use only
• Application notes: WB: The detection limit for ABCB11 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

References

Li, Sisi et al. Dietary Betaine Addition Promotes Hepatic Cholesterol Synthesis, Bile Acid Conversion, and Export in Rats Nutrients, 12, (2020)

PubMed: 32414094

Flow Cytometry analysis of A431 cells using anti-ABCB11 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ABCB11 using anti-ABCB11 antibody.Lane 1:human HCCT tissue; 2:rat liver tissue; 3:mouse liver tissue.

IF analysis of ABCB11 using anti-ABCB11 antibody.ABCB11 was detected in paraffin-embedded section of mouse liver tissues.

IHC analysis of ABCB11 using anti-ABCB11 antibody. ABCB11 was detected in paraffin-embedded section of Mouse Liver Tissue.

IHC analysis of ABCB11 using anti-ABCB11 antibody. ABCB11 was detected in paraffin-embedded section of Rat Liver Tissue.

IHC analysis of ABCB11 using anti-ABCB11 antibody. ABCB11 was detected in paraffin-embedded section of Human Liver Cancer Tissue.