ABCB1 / MDR1 / P Glycoprotein Antibody (clone JSB-1)

• Catalog Number: orb1538594
• Category: Antibodies
• Description: ABCB1 / MDR1 / P Glycoprotein Antibody (clone JSB-1)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: JSB-1
• Tested applications: FC, IHC, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Dilution range: Flo, IHC, IHC-Fr, IHC-P (1:50), WB
• Conjugation: Unconjugated
• Target: ABCB1 / MDR1 / P Glycoprotein
• Storage: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
• Buffer/Preservatives: Tissue Culture Supernatant, 0.1% Sodium Azide, 1% rAlbumin
• Alternative names: ABCB1, ABC20, Abcb1b, CLCS, Colchicin sensitivity,
• Note: For research use only
• Application notes: Further information: JSB-1 has potential value for diagnostic detection of MDR cells in human tumor samples. Working dilutions. Flow cytometry: at least 1:10. Western blotting: start optimizing working dilution a 1:10. Immunocytochemistry: acetone fixed cell preparation at least 1:20. Immunohistochemistry: acetone-fixed frozen sections at least 1:20, formalin-fixed paraffin embedded tissues 1:20. Instructions for use Optimal staining results are obtained with routine 2-step ABC or APAAP methods using acetone-fixed cytocentrifuge preparations or cryostat sections. In case the B-5 fixative is used (see below) also paraffin embedded tissue can be stained with the antibody. In Flow cytometry fixate cells in 10% (v/v) Lysing solution followed by primary antibody and antiMouse-FITC. In developing a Western blot staining pattern use an anti-Mouse-HRP.
• Expiration Date: 12 months from date of receipt.