AAMP CCL18 Antibody

• Catalog Number: orb215878
• Category: Antibodies
• Description: AAMP CCL18 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human AAMP recombinant protein (Position: E235-R434).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 9849 MW
• UniProt ID: P55774
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: C-C motif chemokine 18;Alternative macrophage acti
• Note: For research use only
• Application notes: WB: The detection limit for AAMP is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-AAMP antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of AAMP using anti-AAMP antibody.Lane 1:recombinant human AAMP protein 0.5 ng.

WB analysis of AAMP using anti-AAMP antibody.Lane 1:human A431 cell; 2:human HeLa cell; 3:human HepG2 cell; 4:human MCF-7 cell.

IF analysis of AAMP using anti-AAMP antibody. AAMP was detected in immunocytochemical section of U20S cells.

IHC analysis of AAMP using anti-AAMP antibody. AAMP was detected in a paraffin-embedded section of human mammary cancer tissue.