AAGAB Antibody

• Catalog Number: orb1520790
• Category: Antibodies
• Description: AAGAB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 265 and 315
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 50 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: AAGAB
• UniProt ID: Q6PD74
• NCBI: Q6PD74.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: PPKP1; PPKP1A; p34; KPPP1; alpha- and gamma-adapti
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse AAGAB by western blot. Samples: Whole cell lysate (10 µg) from BeWo, AlphaTC1 Clone 9, and A20 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AAGAB antibody (orb1520790) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human AAGAB by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, U2OS, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AAGAB antibody (orb1520790) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human AAGAB by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AAGAB antibody (orb1520790) used for IP at 1 µg per reaction.