7-Ketocholesterol Antibody

SKU: orb396604

Description

Mouse Anti-7-Ketocholesterol (7-KC) Monoclonal IgG2b

Research Area

Cancer Biology, Cell Biology, Metabolism Research

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Dilution Range: WB (1:1000); ICC/IF (1:50); ELISA (1:1000)
• Reactivity: All
• Application Notes:
  A 1:1000 dilution was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: 70
• Immunogen: Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
• Target: 7-Ketocholesterol
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

7-Ketocholesterol, 7-Keto Cholesterol, 7-Oxocholesterol, Δ5-Cholesterol-3β-ol-7-one, 5-Cholesten-3β-ol-7-one, Cholest-5-en-3β-ol-7-one, 3β-Hydroxy-5-cholesten-7-one, 3-Hydroxy-5-cholestene-7-one, SC-4722

Quick Database Links

Gene Symbol

7-Ketocholesterol