7-Ketocholesterol Antibody (HRP)

SKU: orb396617

Description

Mouse monoclonal antibody against 7-Ketocholesterol conjugated to HRP.

Research Area

Cancer Biology, Cell Biology, Metabolism Research

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Dilution range: WB (1:1000); ICC/IF (1:50); ELISA (1:1000)
• Reactivity: All
• Application Notes:
  A 1:1000 dilution of SMC-512 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: 70
• Immunogen: Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
• Target: 7-Ketocholesterol
• Purification: Protein G Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

7-Ketocholesterol, 7-Keto Cholesterol, 7-Oxocholesterol, Δ5-Cholesterol-3β-ol-7-one, 5-Cholesten-3β-ol-7-one, Cholest-5-en-3β-ol-7-one, 3β-Hydroxy-5-cholesten-7-one, 3-Hydroxy-5-cholestene-7-one, SC-4722

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 7E1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A, C, E, G) - Untreated. (B, D, F, H) - Cells cultured overnight with 50 μM H2O2. (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alexa Fluor 633 F-Actin stain. (E, F) 7-Ketocholesterol Antibody. (G, H) Composite.

Western Blot analysis of 7-Ketocholesterol-BSA Conjugate showing detection of 67 kDa 7-Ketocholesterol protein using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 7E1. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 μg). Lane 3: BSA (2.0 μg). Lane 4: 7-ketocholesterol-BSA (0.5 μg). Lane 5: 7-ketocholesterol-BSA (2.0 μg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Quick Database Links

Gene Symbol

7-Ketocholesterol