6*His, His-Tag Antibody

SKU: orb394654

Description

Mouse monoclonal antibody to 6*His, His-Tag.

Images & Validation

• Tested Applications: ELISA, IF, IP, WB
• Dilution range: WB: 1:1000-1:10000; IP: 1:1000-1:10000; IF: 1:20-1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Target: 6*His, His-Tag
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Protein A+G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Disclaimer: For research use only

Alternative Names

6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag, HISTIDINE TAGGED

Transfected HEK-293 cells using orb394654 (6*His, His-Tag Antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L).

IP Result of anti-6*His, His-Tag (IP: orb394654, 7ug; Detection: orb394654 1:500) with Transfected HEK-293 cells lysate 300 ug.

Western blot of 6*His-tagged fusion protein with orb394654 (anti-6*HIS tag) at dilution of 1:5000.

Quick Database Links

Gene Symbol

6*His, His-Tag