6*His, His-Tag Antibody

• Catalog Number: orb394654
• Category: Antibodies
• Description: Mouse monoclonal antibody to 6*His, His-Tag.
• Target: 6*His, His-Tag
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Protein A+G purified
• Tested applications: ELISA, IF, IP, WB
• Dilution range: WB: 1:1000-1:10000; IP: 1:1000-1:10000; IF: 1:20-1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Transfected HEK-293 cells using orb394654 (6*His, His-Tag Antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L).

IP Result of anti-6*His, His-Tag (IP: orb394654, 7ug; Detection: orb394654 1:500) with Transfected HEK-293 cells lysate 300 ug.

Western blot of 6*His-tagged fusion protein with orb394654 (anti-6*HIS tag) at dilution of 1:5000.