6-Aminofluorescein

SKU: orb1307241

Description

6-Aminofluorescein (6-AF) is a versatile fluorescent dye used as a marker in biochemical research. It is applicable for in vitro labeling and in vivo imaging studies due to its favorable spectral properties, facilitating investigations in cell biology and molecular tracking.

Key Properties

• CAS Number: 51649-83-3
• MW: 347.32
• Purity: 99.26% (May vary between batches)
• Formula: C₂₀H₁₃NO₅
• SMILES: Nc1ccc2C(=O)OC3(c2c1)c1ccc(O)cc1Oc1cc(O)ccc31
• Target: Others
• Solubility: 10% DMSO+40% PEG300+5% Tween-80+45% Saline:3.3 mg/mL (9.5 mM);DMSO:90 mg/mL (259.13 mM)

Bioactivity

• In Vitro:
  Instructions 1. Preparation of reaction system: 1) 6-AF solution: Prepare 6-AF stock solution using appropriate solvent (e.g. DMSO or PBS), usually at a concentration of 1–10 mM. 2) Prepare target molecules (e.g. enzymes or metabolites) in buffer. 3) Add 6-AF as a fluorescent probe, usually at a concentration of 1–100 µM. 2. Detection process: Record fluorescence signal using a fluorometer or microplate reader (excitation wavelength 495 nm, emission wavelength 515 nm). 3. Data analysis: Compare the fluorescence intensity of the experimental group and the control group to evaluate the dynamic changes of the target molecule or reaction.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, 6 Aminofluorescein, 6-AF, 6-Aminofluorescein, 6Aminofluorescein

Compound Identifiers

PubChem CID

103924