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Catalog Number | C311-02 |
---|---|
Category | Tools |
Description | 5min Universal Ligation Mix is a ready-to-use 2 × premix solution, containing T4 DNA ligase that catalyzes the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini in double-strand DNA or RNA. This kit is suitable for sticky end ligation, blunt-end ligation and TA ligation. Buffer with optimized ligation enhancer makes the reaction more efficient and convenient. Ligation can be completed in 5 min at 25°C. The product can be used directly to transform many chemically competent cells. |
Storage | Store at -30 ~ -15°C and transport at ≤0°C. |
Note | For research use only |
Application notes | Compatible: Compatible with sticky ends, blunt ends, TA , linker or adapter ligation Fast: Ligation can be completed quickly in 5 minutes at 25°C Convenient: Reaction mixture can be used directly for bacterial transformation |
Expiration Date | 12 months from date of receipt. |
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